Simultaneous measurement of pulmonary diffusing capacity for carbon monoxide and nitric oxide

In Europe and America, the newly-developed, simultaneous measurement of diffusing capacity for CO (D_LCO) and NO (D_LNO) has replaced the classic D_LCO measurement for detecting the pathophysiological abnormalities in the acinar regions. However, simultaneous measurement of D_LCO and D_LNO is currently not used by Japanese physicians. To encourage the use of D_LNO in Japan, the authors reviewed aspects of simultaneously-estimated D_LCO and D_LNO from previously published manuscripts. The simultaneous D_LCO-D_LNO technique identifies the alveolocapillary membrane-related diffusing capacity (membrane component, D_M) and the blood volume in pulmonary microcirculation (V_C); V_C is the principal factor constituting the blood component of diffusing capacity (D_B,D_B=θ·V_C where θ is the specific gas conductance for CO or NO in the blood). As the association velocity of NO with hemoglobin (Hb) is fast and the affinity of NO with Hb is high in comparison with those of CO, θ_NO can be taken as an invariable simply determined by diffusion limitation inside the erythrocyte. This means that θ_NO is independent of the partial pressure of oxygen (PO₂). However, θ_CO involves the limitations by diffusion and chemical reaction elicited by the erythrocyte, resulting in θ_CO to be a PO₂-dependent variable. Furthermore, D_LCO is determined primarily by D_B (∼77%), while D_LNO is determined equally by D_M (∼55%) and D_B (∼45%). This suggests that D_LCO is more sensitive for detecting microvascular diseases, while D_LNO can equally identify alveolocapillary membrane and microcirculatory abnormalities.