Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

32.6K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.6K
Embryonic Stem Cells00:57

Embryonic Stem Cells

5.2K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.2K
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

97.8K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
97.8K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Structures of Carboxylic Acid Derivatives01:28

Structures of Carboxylic Acid Derivatives

3.8K
Structure of Carboxylic Acid Derivatives
Carboxylic acid derivatives contain an acyl group attached to a heteroatom such as chlorine, oxygen, or nitrogen. The carbonyl carbon and oxygen are both sp2-hybridized with an unhybridized p orbital.
The three sp2 orbitals of the carbonyl carbon form three σ bonds, one each with the carbonyl oxygen, the α carbon, and the heteroatom, whereas the other two sp2 orbitals of the carbonyl oxygen are occupied by the lone pairs. Further, the unhybridized p...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dose-Dependent Effects of Soy Lecithin Intake on Synaptic Ultrastructure in Brain Neurons and Behavioral Patterns of C57BL/6 Laboratory Mice.

Biochemistry. Biokhimiia·2026
Same author

Highly Selective Interfacial Route to Eight-Functional Sucrose Methacrylate for Biocompatible Scaffold Fabrication.

Polymers·2026
Same author

Human-in-the-Loop Enhances Machine Learning Inference in Intraoperative Optical Coherence Tomography Glioma Imaging.

Medical sciences (Basel, Switzerland)·2026
Same author

Specific elimination of m.8993T>G mitochondrial haplotype in NARP cybrid cells by CRISPR-Cas9 system.

Scientific reports·2026
Same author

Integrative Study of the Life Cycle in the Marine Protist <i>Thraustochytrium aureum</i> ssp. <i>strugatskii</i>.

International journal of molecular sciences·2025
Same author

The olfactory epithelium as a gateway for bloodborne nanoparticles to the central nervous system.

Biomaterials science·2025

Related Experiment Video

Updated: Feb 13, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

14.0K

Mitochondria structural reorganization during mouse embryonic stem cell derivation.

Lyubov A Suldina1, Ksenia N Morozova2,3, Aleksei G Menzorov1,4

  • 1Institute of Cytology and Genetics SB RAS, Russian Academy of Sciences, Lavrentiev ave., 10, Novosibirsk, Russia, 630090.

Protoplasma
|March 18, 2018
PubMed
Summary

This study details the ultrastructural changes in mouse embryonic stem (ES) cells during in vitro derivation. We observed significant cellular reorganization, particularly in mitochondria, as inner cell mass (ICM) cells transform into stable ES cells.

Keywords:
Cell ultrastructureElectron microscopyEmbryonic stem cellMitochondria

More Related Videos

Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

34.9K
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
08:27

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

Published on: October 14, 2014

11.7K

Related Experiment Videos

Last Updated: Feb 13, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

14.0K
Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

34.9K
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
08:27

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

Published on: October 14, 2014

11.7K

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Stem Cell Research

Background:

  • Mouse embryonic stem (ES) cells are crucial for developmental biology and transgenic research.
  • The ultrastructural reorganization of inner cell mass (ICM) cells during in vitro culture is not well-described.

Purpose of the Study:

  • To perform comparative morphological and morphometric analyses of ES cell lines during in vitro derivation.
  • To detail the ultrastructural reorganization of ICM cells into ES cells.

Main Methods:

  • Comparative morphological and morphometric analyses of three ES cell lines.
  • Examination of blastocyst ICM cells and ES cells at different passages (0, 2, 4, and 15).
  • Ultrastructural analysis focusing on cellular components, including mitochondria.

Main Results:

  • At passage 0, cultures contained ES-like cells, fibroblast-like cells (trophoblast derivatives), and dying cells.
  • ES-like cells at passage 0 exhibited autolysosomes and enlarged mitochondria with varied morphology.
  • ES cell morphology and parameters stabilized by passage 4, showing reduced heterogeneity.

Conclusions:

  • ICM cell structures destabilize during ES cell line derivation and subsequently reorganize into typical ES cell structures.
  • Mitochondrial ultrastructure suggests functional changes occur during early ES cell formation from the ICM.