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Medicinal mushroom Phellinus igniarius induced cell apoptosis in gastric cancer SGC-7901 through a

Fei-Fei Wang1, Chao Shi1, Yue Yang1

  • 1School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|March 18, 2018
PubMed

Insights

Phellinus igniarius extract shows potent antitumor effects against gastric cancer cells. It induces apoptosis through a mitochondria-dependent pathway, offering potential as a natural therapeutic agent.

Area of Science:

  • Oncology
  • Pharmacology
  • Natural Products Research

Background:

  • Gastric cancer (GC) is a leading cause of cancer death globally.
  • Phellinus igniarius is a traditional East Asian medicinal mushroom with reported anti-cancer properties.

Purpose of the Study:

  • To evaluate the anti-tumor activity of Phellinus igniarius extract.
  • To elucidate the underlying mechanisms of its anti-cancer effects.

Main Methods:

  • MTT assay for in vitro cytotoxicity.
  • Xenograft nude mouse model for in vivo tumor growth inhibition.
  • Flow cytometry and Western blotting to analyze apoptosis and cell cycle.

Main Results:

  • Total ethanol extract of Phellinus igniarius (TPI) exhibited cytotoxicity against multiple cancer cell lines, notably gastric cancer SGC-7901.
  • TPI significantly inhibited tumor growth in vivo and induced apoptosis in SGC-7901 cells.
  • TPI triggered mitochondrial-dependent apoptosis by down-regulating cyclin D1, collapsing mitochondrial membrane potential, activating caspases, and altering Bax/Bcl-2 ratio.

Conclusions:

  • Phellinus igniarius extract demonstrates significant anti-tumor activity against gastric cancer.
  • The extract induces apoptosis via a mitochondria-dependent pathway, suggesting its potential as a natural therapeutic agent for gastric cancer prevention and treatment.
Keywords:
Antitumor activityApoptosisGastric cancerMitochondria-dependent pathwayPhellinus igniariusSGC-7901

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