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Updated: Feb 13, 2026

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
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Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

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Gefitinib inhibits retina angiogenesis by affecting VEGF signaling pathway

Haitao Hu1, Lanxiang Hao2, Biao Yan3

  • 1The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, 210029, China; Eye Hospital of Nanjing Medical University, 138# Han-Zhong Road, Nanjing 210029, China; Yancheng City No. 1 People's Hospital, Yancheng, 224001, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|March 19, 2018
PubMed

Insights

Gefitinib significantly reduces retinal angiogenesis in a mouse model by inhibiting the VEGF signaling pathway. This finding suggests Gefitinib

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Pharmacology

Background:

  • Retinal angiogenesis contributes to vision-threatening diseases.
  • Gefitinib is an oral antiangiogenic agent, but its effect on retinal angiogenesis is unknown.

Purpose of the Study:

  • To investigate Gefitinib's effect on oxygen-induced retinal angiogenesis.
  • To explore the correlation between Gefitinib and the VEGF signaling pathway.

Main Methods:

  • Oxygen-induced retinopathy mouse models were established.
  • Mice were treated with Gefitinib or DMSO.
  • Retinal vascular morphology was assessed using Ink, HE, and isolectin staining.
  • VEGF, Cyclin E, CDK2, and CD31 expression levels were measured.

Main Results:

  • Oxygen exposure increased retinal neovascularization and vaso-obliteration.
  • Gefitinib treatment significantly reduced newborn retinal vessels.
  • Gefitinib affected the VEGF/Cyclin E/CDK2/CD31 signaling pathway.

Conclusions:

  • Gefitinib effectively inhibits retinal angiogenesis in a mouse model.
  • The antiangiogenic effect of Gefitinib is linked to the VEGF/Cyclin E/CDK2/CD31 pathway.
  • Gefitinib shows potential for treating retina angiogenesis.
Keywords:
AngiogenesisGefitinibOxygen

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