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Effect of Centrifuge Temperature on Routine Coagulation Tests
Hayrullah Yazar1, Fatma Özdemir2, Elif Köse3
1Department of Medical Biochemistry, Sakarya University Faculty of Medicine, Adapazan, Turkey.
Acta Haematologica
|March 19, 2018
Summary
Centrifuge temperature significantly impacts coagulation test results. Cooled centrifugation (2-4°C) yielded different values for prothrombin time, INR, aPTT, fibrinogen, and D-dimer compared to standard centrifugation (25°C).
Area of Science:
- Clinical Laboratory Science
- Hematology
- Diagnostic Testing
Background:
- Centrifugation temperature is a critical pre-analytical variable in laboratory testing.
- Standard centrifugation (25°C) and cooled centrifugation (2-4°C) may influence coagulation assay outcomes.
Purpose of the Study:
- To investigate the impact of centrifugation temperature on key coagulation test results.
- To compare coagulation parameters obtained using standard versus cooled centrifugation methods.
Main Methods:
- Blood samples from 771 participants were collected and divided for standard (25°C) and cooled (2-4°C) centrifugation.
- Prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer were measured.
- Tests were performed using identical reagent lot numbers and coagulation analyzers.
Main Results:
- Significant differences (p < 0.001) were observed in PT, INR, aPTT, fibrinogen, and D-dimer values between standard and cooled centrifugation.
- Prothrombin time was notably higher in participants on anticoagulant therapy (p < 0.018).
- Specific median differences included: PT (10.30 vs 10.50 s), INR (1.04 vs 1.09), aPTT (28.90 vs 29.40 s), fibrinogen (321.5 vs 322.1 mg/dL), and D-dimer (179.5 vs 168.7 µg FEU/mL).
Conclusions:
- Centrifuge temperature significantly affects coagulation test results.
- Further disease-specific studies are warranted to elucidate the clinical implications of these temperature-dependent variations.
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