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Updated: Feb 13, 2026

High-throughput Yeast Plasmid Overexpression Screen
Published on: July 27, 2011
Simultaneous Overexpression of Integrated Genes by Copy Number Amplification of a Mini-Yeast Artificial Chromosome
Heo-Myung Jung1, Yeon-Hee Kim1,2
1Department of Smart Bio-Health, Dong-Eui University, Busan 47340, Republic of Korea.
Abstract:
A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and a 184-kb split-YAC. The 44-kb split-mini YAC was amplified a maximum of 9-fold, and the activity of the reporter enzymes integrated into the split-mini YAC increased about 5-7-fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number.
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