Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

24.4K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.4K
Plant Cell Wall02:43

Plant Cell Wall

60.6K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
60.6K
Plant Hormones01:56

Plant Hormones

27.7K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
27.7K
Bioequivalence Experimental Study Designs: Completely Randomized and Randomized Block Designs01:20

Bioequivalence Experimental Study Designs: Completely Randomized and Randomized Block Designs

256
Body:Bioequivalence experimental study designs are crucial methodologies used in evaluating and comparing the bioavailability of different drug products. These designs are categorized into various types: completely randomized, randomized block, repeated measures, cross and carry-over, and Latin square designs.Completely randomized designs involve randomly allocating treatments to all subjects participating in the experiment. This allocation is achieved by assigning unique random numbers to...
256
Meristems and Plant Growth02:36

Meristems and Plant Growth

49.7K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lost and found: Rediscovery and genomic characterization of sowthistle yellow vein virus after a 30+ year hiatus.

Virus research·2020
Same author

Diversity and epidemiology of plant rhabdoviruses.

Virus research·2020
Same author

Characterization of <i>Soybean yellow shoot virus</i>, a New Member of the Family <i>Potyviridae</i> Infecting Soybean Plants in Brazil.

Plant disease·2019
Same author

Dichorhaviruses in their Host Plants and Mite Vectors.

Advances in virus research·2018
Same author

Development of Model Systems for Plant Rhabdovirus Research.

Advances in virus research·2018
Same author

The Rise and Rise of Nicotiana benthamiana: A Plant for All Reasons.

Annual review of phytopathology·2018

Related Experiment Video

Updated: Feb 13, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
10:28

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Published on: October 19, 2018

22.3K

Protein Localization and Interaction Studies in Plants: Toward Defining Complete Proteomes by Visualization.

Michael M Goodin1

  • 1University of Kentucky, Lexington, KY, United States.

Advances in Virus Research
|March 20, 2018
PubMed
Summary

This study outlines methods for studying protein interactions and localization in plants, crucial for understanding virus-plant interactions. It details microscopy techniques and experimental controls for accurate results in plant virology research.

Keywords:
ColocalizationConfocal microscopyNicotiana benthamianaProtein interaction

More Related Videos

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.6K
Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

14.6K

Related Experiment Videos

Last Updated: Feb 13, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
10:28

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Published on: October 19, 2018

22.3K
Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.6K
Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

14.6K

Area of Science:

  • Plant biology
  • Virology
  • Microscopy and imaging techniques

Background:

  • Understanding virus-plant interactions requires studying protein localization and interactions within plant cells.
  • Nicotiana benthamiana is a key model organism for transient expression assays in plant virology.
  • Laser-scanning confocal microscopy is a primary tool for examining these interactions.

Purpose of the Study:

  • To provide a comprehensive workflow for protein interaction and localization experiments in plants.
  • To introduce basic microscopy principles for researchers new to imaging techniques.
  • To detail specific imaging-based experiments and emphasize data interpretation.

Main Methods:

  • Transient expression assays in Nicotiana benthamiana leaves.
  • Laser-scanning confocal microscopy for visualization.
  • Autofluorescent protein tagging for protein localization.
  • Colocalization studies to assess protein proximity.
  • Bimolecular fluorescence complementation (BiFC) for interaction detection.

Main Results:

  • A detailed workflow for conducting protein localization and interaction studies is presented.
  • Key imaging techniques including protein localization, colocalization, and BiFC are explained.
  • Emphasis is placed on the importance of controls and accurate data interpretation.

Conclusions:

  • Standardized methods for protein localization and interaction studies enhance mechanistic understanding of virus-plant interactions.
  • The chapter equips researchers with essential imaging techniques and interpretation guidelines.
  • The need for large-scale, proteome-wide localization projects, particularly for nuclear proteins, is highlighted.