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Updated: Feb 13, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
Antagonistic Bacillus spp. reduce blast incidence on rice and increase grain yield under field conditions
Afroz Rais1, Muhammad Shakeel1, Kamran Malik1
1Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan.
Abstract:
Rice blast is a severe threat for agricultural production. Plant growth promoting rhizobacteria could be suitable biocontrol agents to reduce the disease incidence. In this study, Bacillus spp. KFP-5, KFP-7, KFP-17 significantly reduced disease severity by 40-52% with grain yield of 3.2-3.9 t ha-1 in two rice varieties i.e., basmati super and basmati 385. Bacillus spp. significantly colonized the rice rhizosphere with a cell population of 2.40E+06-5.6E+07CFU. Rice plants treated with antagonistic bacterial suspension followed by challenge inoculation with P. oryzae were found to have higher activities of antioxidant enzymes such as superoxide dismutase (308-266 Ug-1 FW), peroxidase (change in absorbance (ΔA) = 0.20-0.71 min-1 g-1 FW), polyphenol oxidase (ΔA = 0.29-0.58 min-1 g-1 FW) and phenylalanine ammonia lyase (ΔA = 0.32-0.59 min-1 g-1 FW). A consistency in the performance of strains was observed in the consecutive years 2013-2014. These findings suggest that indigenous Bacillus spp. could be a potential bio-inoculum for rice to control blast diseases and enhance yield.
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