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Updated: Feb 13, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
Published on: April 22, 2013
Investigation of albumin-derived perfluorocarbon-based capsules by holographic optical trapping
Jannis Köhler1, Jegor Ruschke1, Katja Bettina Ferenz2
1Applied Laser Technologies, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
Abstract:
Albumin-derived perfluorocarbon-based capsules are promising as artificial oxygen carriers with high solubility. However, these capsules have to be studied further to allow initial human clinical tests. The aim of this paper is to provide and characterize a holographic optical tweezer to enable contactless trapping and moving of individual capsules in an environment that mimics physiological (in vivo) conditions most effectively in order to learn more about the artificial oxygen carrier behavior in blood plasma without recourse to animal experiments. Therefore, the motion behavior of capsules in a ring shaped or vortex beam is analyzed and optimized on account of determination of the optical forces in radial and axial direction. In addition, due to the customization and generation of dynamic phase holograms, the optical tweezer is used for first investigations on the aggregation behavior of the capsules and a statistical evaluation of the bonding in dependency of different capsule sizes is performed. The results show that the optical tweezer is sufficient for studying individual perfluorocarbon-based capsules and provide information about the interaction of these capsules for future use as artificial oxygen carriers.
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