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Updated: Feb 13, 2026

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
Published on: June 21, 2011
Quantification of Tumor Material Uptake
Richard N Hanna1,2, Catherine C Hedrick2
1Department of Respiratory, Inflammation and Autoimmune Diseases, MedImmune, LLC, Gaithersburg, USA.
Abstract:
Extracellular tumor material including exosomes, microvesicles and apoptotic tumor debris may help cancers invade new organs. Enhancing the removal of extracellular tumor material by immune cells represents a novel immunotherapy approach for preventing cancer metastasis. This protocol quantifies the uptake and removal of extracellular tumor material from circulation and tissues by immune cells. In this assay fluorescent tumor cells are transferred into mice, and then immune cells are quantified by either flow cytometry or imaging cytometry for their uptake of tumor material.
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