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Updated: Feb 13, 2026

Imaging FITC-dextran as a Reporter for Regulated Exocytosis
Published on: June 20, 2018
High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells
Xingmin Aaron Zhang1,2, Thomas F J Martin2
1Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, WI, USA.
Abstract:
Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay using DCV cargo, NPY-Venus, and an orthogonal 3H-serotonin secretion assay. The NPY-Venus secretion assay was successfully used for a high throughput siRNA screen, and the serotonin secretion assay was used to validate hits identified from the screen (Sorensen, 2017; Zhang et al., 2017).
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