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Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
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Infectious Subviral Particle-induced Hemolysis Assay for Mammalian Orthoreovirus

Anthony J Snyder1, Pranav Danthi1

  • 1Department of Biology, Indiana University, Bloomington, Indiana, USA.

Bio-Protocol
|March 20, 2018
PubMed

Insights

Mammalian orthoreovirus uses pore-forming peptides to enter cells, a crucial step for genome delivery. This study details an in vitro assay to measure reovirus-induced pore formation during viral entry.

Area of Science:

  • Virology
  • Molecular Biology
  • Biophysics

Background:

  • Mammalian orthoreovirus (reovirus) is a virus that requires membrane penetration to infect host cells.
  • Pore-forming peptides are essential for reovirus to breach the cell membrane and deliver its genetic material.
  • Understanding viral entry mechanisms is critical for developing antiviral strategies.

Purpose of the Study:

  • To describe a standardized in vitro assay for quantifying reovirus-induced pore formation.
  • To provide a method for studying the biophysical mechanisms of reovirus membrane penetration.

Main Methods:

  • Development of an in vitro assay to measure pore formation.
  • Utilizing techniques to monitor membrane permeabilization induced by reovirus.

Main Results:

  • The assay successfully measures reovirus-induced pore formation.
  • The protocol allows for quantitative analysis of reovirus's membrane-disrupting activity.

Conclusions:

  • The described in vitro assay is a valuable tool for studying reovirus entry.
  • This method facilitates research into the role of pore-forming peptides in viral pathogenesis.
Keywords:
HemolysisMammalian orthoreovirusMembrane penetrationReoviridaeViral entryVirology

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