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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
Published on: October 30, 2014
Obtaining Acute Brain Slices
Thomas Papouin1, Philip G Haydon1
1Department of Neuroscience, Tufts University School of Medicine, Boston, MA, USA.
Abstract:
Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the dissection, extraction and acute slicing of the mouse brain, including tips and list of material required.
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