Rb interactome data and its modulations during cell cycle progression in HEK 293 cells

Area of Science:

• Molecular Biology
• Cell Biology
• Proteomics

Background:

• The Retinoblastoma (Rb) protein is a critical tumor suppressor.
• Rb regulates the G1S cell cycle checkpoint, preventing uncontrolled cell division.
• Detailed knowledge of Rb's dynamic interactome across the cell cycle is limited.

Purpose of the Study:

• To comprehensively identify Rb-interacting proteins during different cell cycle phases (G0, G1, S, G2).
• To provide a dataset of Rb interactors in HEK 293 cells.
• To elucidate Rb's role in cell cycle regulation through its binding partners.

Main Methods:

• Affinity purification coupled with mass spectrometry (AP-MS) was used to isolate Rb-protein complexes.
• Stable Isotope Labeling by Amino acids in Cell culture (SILAC) was employed for quantitative proteomic analysis across cell cycle stages.
• Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and protein pilot software were used for data analysis.

Main Results:

• A comprehensive dataset of Rb interacting partners was generated across G0, G1, S, and G2 phases.
• Quantitative data revealed dynamic changes in Rb's protein interaction network throughout the cell cycle.
• The dataset provides insights into the regulation of the G1S checkpoint by Rb.

Conclusions:

• This study presents a detailed map of the Rb interactome across the cell cycle.
• The findings enhance our understanding of Rb's function in cell cycle control.
• The data is publicly available for further research in cell cycle regulation and oncology.