Related Experiment Video

Updated: Feb 13, 2026

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
11:58

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Published on: January 6, 2023

3.4K

The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive

Yuta Kasagi1, Prasanna M Chandramouleeswaran2,3, Kelly A Whelan2,3

  • 1Division of Pediatric Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania.

Cellular and Molecular Gastroenterology and Hepatology
|March 20, 2018
PubMed
Abstract

Keywords:
3D, 3-dimensionalBCH, basal cell hyperplasiaDAPI, 4′,6-Diamidino-2-Phenylindole, DihydrochlorideDNMAML1, dominant negative MAML1DOX, doxycyclineEGF, epidermal growth factorEMT, epithelial-mesenchymal transitionEoE, eosinophilic esophagitisEosinophilic EsophagitisGERD, gastroesophageal reflux diseaseGFP, green fluorescent proteinGSI, γ-secretase inhibitorH&E, hematoxylin and eosinIF, immunofluorescenceIHC, immunohistochemistryIL, interleukinIVL, InvolucrinKSFM, keratinocyte SFMKSFMC, KSFM containing 0.6 mM Ca2+KeratinocytesMAML1, Mastermind-like protein1OFR, organoid formation rateSquamous Cell DifferentiationTNF-α, tumor necrosis factor-αThree-DimensionalTslp, thymic stromal lymphopoietinaDMEM/F12, advanced Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12qRT-PCR, quantitative reverse-transcription polymerase chain reaction

More Related Videos

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids10:43

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Published on: December 23, 2022

4.1K
Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function09:40

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

7.4K

Related Concept Videos

Epithelial Tissues and Their Functions01:23

Epithelial Tissues and Their Functions

Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium.
Epithelial tissues provide the body's first line of protection from physical,...
41.2K
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Esophageal Varices-I: Introduction01:24

Esophageal Varices-I: Introduction

Esophageal varices are dilated, tortuous veins which are found mainly in the submucosa of the lower esophagus but which may also appear higher up or extend into the stomach. They develop due to increased pressure in the portal venous system, often as a result of liver cirrhosis. This condition scars and damages the liver, impeding normal blood flow through the portal vein. To compensate, blood seeks alternative pathways, forming fragile new vessels (varices) in the esophagus and stomach. These...
1.5K
Esophageal Perforation-I: Introduction01:22

Esophageal Perforation-I: Introduction

Esophageal perforation is a severe medical condition characterized by a breach in the integrity of the esophageal wall. This breach can occur due to various factors such as trauma, medical procedures, or underlying diseases. When the esophageal wall is compromised, it allows food, fluids, and digestive juices into the chest cavity or adjacent structures, leading to potential complications and health risks.
The location of esophageal perforation can vary, occurring anywhere along the esophagus....
622
Esophageal Strictures-I: Introduction01:30

Esophageal Strictures-I: Introduction

Esophageal strictures involve abnormal narrowing or tightening of the esophagus. They vary in length and severity, ranging from mild constriction to complete obstruction, and are classified as benign (noncancerous) or malignant (cancerous).
Etiology
The primary cause of esophageal strictures is long-standing gastroesophageal reflux disease (GERD), accounting for about 70 to 80% of adult cases. Chronic acid reflux can lead to injury and scarring of the esophageal lining, culminating in...
911
Cross-reactivity00:42

Cross-reactivity

Overview
33.1K