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A Mouse Model of Intestinal Partial Obstruction
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A Mouse Model of Intestinal Partial Obstruction.

Se Eun Ha1, Lai Wei1, Brian G Jorgensen1

  • 1Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine.

Journal of Visualized Experiments : Jove
|March 20, 2018
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Summary

A new mouse model for intestinal obstruction surgery offers a reliable method to study cellular changes in the gut. This partial obstruction (PO) model has low mortality and induces smooth muscle hypertrophy for research.

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Area of Science:

  • Gastroenterology
  • Surgical Pathology
  • Animal Models

Background:

  • Intestinal obstructions, caused by adhesions or tumors, impede peristalsis, but cellular remodeling mechanisms remain unclear.
  • Existing mouse models for intestinal obstruction have high mortality or fail to induce necessary tissue changes like hypertrophy.
  • Understanding these cellular mechanisms is crucial for treating gastrointestinal diseases.

Purpose of the Study:

  • To describe a detailed and reproducible protocol for surgical induction of partial intestinal obstruction (PO) in mice.
  • To establish a cost-effective and time-efficient mouse model with low mortality for studying intestinal obstruction.
  • To provide a model for investigating cellular remodeling, including smooth muscle hypertrophy, in response to obstruction.

Main Methods:

  • Surgical implantation of a silicone ring around the ileum to create a partial obstruction in mice.
  • Monitoring of mortality rates and development of intestinal dilation and smooth muscle hypertrophy over two weeks.
  • Utilizing the model to study pathological changes in intestinal smooth muscle cells (SMC), interstitial cells of Cajal (ICC), PDGFRα+, and neuronal cells.

Main Results:

  • The described protocol reliably produces reproducible intestinal obstructions with a significantly low mortality rate.
  • The partial blockage induces intestinal dilation and progressive smooth muscle hypertrophy on the oral side of the obstruction.
  • The model successfully mimics key pathological changes observed in human intestinal obstructions.

Conclusions:

  • The surgical PO mouse model provides a valuable in vivo tool for studying the pathophysiology of intestinal obstruction.
  • This model facilitates research into cellular remodeling, including smooth muscle hypertrophy, in response to partial intestinal blockage.
  • The protocol's reliability and low mortality make it suitable for investigating treatments and understanding disease progression in gastrointestinal disorders.