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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
Published on: February 19, 2018
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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
Holger Fehlauer1, Adam L Nekimken2, Anna A Kim2
1Department of Molecular and Cellular Physiology, Stanford University.
Journal of Visualized Experiments : Jove
|March 20, 2018
Summary
Mechanobiology research is advanced by a new device enabling precise mechanical stress application and imaging of cellular responses in live C. elegans. This tool overcomes limitations of previous methods for studying mechanotransduction in vivo.
Area of Science:
- Mechanobiology
- Cellular Mechanotransduction
- Microfluidics
Background:
- Understanding the impact of mechanical stress on cellular function is a key goal in mechanobiology.
- Existing tools struggle to simultaneously deform tissues/cells, image live animals, and immobilize mobile organisms like C. elegans.
- Previous microfluidic devices for C. elegans immobilization limit physical access for mechanical force application.
Purpose of the Study:
- To develop a novel microfluidic device for applying controlled mechanical stress to individual cells in live C. elegans.
- To enable high-resolution imaging of cellular activity, specifically neuronal activation, in response to mechanical stimuli.
- To overcome limitations of prior methods by allowing direct mechanical force delivery and electrophysiological recordings.
Main Methods:
- A microfluidic device integrating pneumatic actuators with a worm-trapping design compatible with high-resolution fluorescence microscopy was created.
- A thin polydimethylsiloxane (PDMS) diaphragm separates an actuation channel from the worm-trapping channel, deflecting to apply force.
- The method was demonstrated using C. elegans strains expressing the calcium indicator GCaMP6s in touch receptor neurons (TRNs).
Main Results:
- The device successfully applies mechanical force to target individual mechanosensitive neurons in C. elegans.
- High-resolution imaging of neuronal activation using genetically-encoded calcium indicators was achieved.
- The system allows for direct physical access for mechanical stimulation and potential electrophysiological recordings.
Conclusions:
- This novel microfluidic device provides a powerful platform for studying mechanobiology in C. elegans.
- The method enables precise mechanical stimulation and real-time imaging of cellular responses in vivo.
- The technology is adaptable for studying various mechanically-sensitive cells and sensors beyond TRNs and calcium indicators.
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