Chemoattractant-Mediated Preference of Non-Self Eggs in Ciona robusta Sperm

Self-fertilization in hermaphroditic species might or might not be advantageous based on the level of inbreeding or outbreeding depression and the opportunity to outcross. This study examined whether chemoattractants can influence selfing rates through changes in sperm swimming behavior in the hermaphroditic tunicate Ciona robusta. The first set of experiments tested sperm preference in a dichotomous choice chamber by allowing the sperm to choose between wells with no eggs and wells with eggs, while the second experiment gave sperm a choice between self eggs and non-self eggs from another C. robusta individual. We found that sperm were about 5 times more likely to be captured in wells with eggs than in empty wells (P < 0.001) and that they were about 1.6 times more likely to be captured in wells with non-self eggs than in those with self eggs (P = 0.002). Additionally, we found that although sperm were activated by water pretreated with eggs, there was no difference in sperm swimming speed and motility in water treated with pooled-egg water compared to self-egg-treated water (P = 0.636 and P = 0.854, respectively). Our results indicate that while chemoattractant identity does not affect the basic mechanics of sperm activation and thus fertilization ability, it can cause sperm to aggregate near non-self eggs in greater numbers. This may allow for sperm to fertilize non-self eggs in greater numbers when available while still retaining the ability to fertilize self eggs.