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A comprehensive proteomics study on platelet concentrates: Platelet proteome, storage time and Mirasol pathogen

Vishal Salunkhe1, Iris M De Cuyper1, Petros Papadopoulos2

  • 1a Department of Blood Cell Research , Sanquin Research and Landsteiner Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA) , Amsterdam , The Netherlands.

Platelets
|March 20, 2018
PubMed

Insights

Pathogen inactivation technologies (PITs) like Mirasol PRT may alter platelet function and accelerate storage lesion. Proteomics analysis revealed specific proteome changes related to Mirasol PRT, distinct from the storage lesion, offering unbiased platelet phenotyping.

Area of Science:

  • * Transfusion Medicine
  • * Proteomics
  • * Blood Safety

Background:

  • * Platelet concentrates (PCs) are essential but face safety concerns due to bacterial contamination risk during storage.
  • * Pathogen inactivation technologies (PITs) enhance blood product safety against various pathogens.
  • * Mirasol Pathogen Reduction Technology (PRT) is a PIT, but concerns exist regarding its impact on platelet quality and potential acceleration of platelet storage lesion (PSL).

Purpose of the Study:

  • * To investigate the proteomic changes in PCs treated with Mirasol PRT and compare them to control PCs during storage.
  • * To determine if Mirasol PRT accelerates or exacerbates the platelet storage lesion (PSL) at a molecular level.
  • * To differentiate proteomic alterations caused by Mirasol PRT from those associated with standard PSL.

Main Methods:

  • * Comprehensive proteomics analysis using a gel-free, label-free quantification (LFQ) approach.
  • * Comparison of Control PCs and Mirasol PRT-treated PCs at multiple storage time points (days 1, 2, 6, and 8).
  • * Protein quantification based on LFQ signal intensities analyzed via MaxQuant/Perseus software.

Main Results:

  • * Marginal overall differences were observed between Mirasol PRT-treated and control PCs.
  • * Significant proteomic changes were identified, specifically linked to known functional alterations induced by Mirasol PRT.
  • * The observed proteomic effects of Mirasol PRT were not solely attributable to an accelerated or enhanced PSL.

Conclusions:

  • * Semi-quantitative proteomics can effectively distinguish proteome changes induced by Mirasol PRT from those of PSL.
  • * Mirasol PRT induces specific proteomic alterations in platelets, independent of PSL.
  • * Proteomics is a valuable, unbiased methodology for phenotyping platelets under various conditions, including PIT treatment.
Keywords:
Blood safetypathogen inactivation technologiesplatelet function testsplatelet transfusionproteomicsstorage time

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