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The effect of storage delay and storage temperature on orthopaedic surgical samples contaminated by Staphylococcus

Maïté Van Cauter1, Olivier Cornu1, Jean-Cyr Yombi1,2

  • 1Department of Orthopedic Surgery, Cliniques Universitaires Saint Luc, Brussels, Belgium.

Plos One
|March 20, 2018
PubMed
Abstract

Insights

Timely processing and refrigeration of samples are crucial for detecting Staphylococcus epidermidis in prosthetic joint infections. Prompt inoculation within 2 hours and storage at 4°C enhance bacterial detection rates.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Orthopedic Surgery

Background:

  • Prosthetic Joint Infection (PJI) poses significant morbidity and mortality risks.
  • Accurate identification of causative microorganisms is vital for effective treatment.
  • Current culture methods for PJI diagnosis have limitations, with 14% of intra-articular punctures yielding negative results.

Purpose of the Study:

  • To evaluate the impact of culture lead time and sample storage temperature on pathogen detection.
  • To optimize diagnostic protocols for Prosthetic Joint Infection (PJI).

Main Methods:

  • Bone fragments from femoral heads during primary arthroplasty were used.
  • Samples were intentionally contaminated with Staphylococcus epidermidis.
  • Four experimental conditions varying storage delay and temperature were tested.

Main Results:

  • Cultivating samples as soon as possible, ideally within 2 hours of collection, is essential.
  • Storing samples at 4°C (refrigeration) positively influences bacterial viability.
  • These findings are specific to Staphylococcus epidermidis and require further investigation for other bacteria.

Conclusions:

  • Prompt sample processing and refrigeration at 4°C improve the detection of Staphylococcus epidermidis in PJI diagnostics.
  • Further research is needed to confirm these findings for a broader range of bacteria implicated in PJI.

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