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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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Structure and Function of Platelets01:18

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The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
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Exponential Functions with Base e01:30

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Exponential functions with base e are essential for modeling continuous processes of growth and decay. The constant e, approximately 2.718, naturally arises in systems where change occurs proportionally to the current value. A positive exponent represents continuous growth, while a negative exponent represents continuous decay. These functions are especially useful for describing situations where change happens smoothly over time rather than in discrete steps.One clear example of exponential...
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Formation of the Platelet Plug01:22

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Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
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Toward Flow Cytometry Based Platelet Function Diagnostics.

Ivar van Asten1,2,3, Roger E G Schutgens3, Rolf T Urbanus1

  • 1Department of Clinical Chemistry and Haematology, Center for Circulatory Health, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.

Seminars in Thrombosis and Hemostasis
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Summary

Flow cytometry offers a promising approach for diagnosing mild platelet function disorders and those with low platelet counts, overcoming limitations of traditional lab tests. Further clinical validation is needed before routine use.

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Area of Science:

  • Hematology
  • Clinical Diagnostics
  • Immunology

Background:

  • Traditional platelet function tests (aggregation, secretion assays) have limitations in diagnosing mild disorders or those with low platelet counts.
  • Inherited platelet function disorders require accurate diagnostic methods for effective management.

Purpose of the Study:

  • To review the applications of flow cytometry in diagnosing platelet function disorders.
  • To discuss the challenges in validating and implementing new diagnostic tools for platelet function testing.

Main Methods:

  • Review of current literature on flow cytometry for platelet function assessment.
  • Analysis of existing diagnostic methods and their limitations.

Main Results:

  • Flow cytometry requires minimal whole blood volume and can assess platelet function in thrombocytopenic samples.
  • Flow cytometry assays have been extensively used in research settings, indicating potential for clinical diagnostics.

Conclusions:

  • Flow cytometry presents a valuable addition to the diagnostic work-up of platelet function disorders.
  • Clinical validation is essential for adopting flow cytometry as a routine diagnostic tool, especially given the lack of a gold standard for mild cases.