Related Experiment Video
Updated: Feb 13, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
miRNA Profiling of Exosomes from Spontaneous Hypertensive Rats Using Next-Generation Sequencing
Xiaoyan Liu1,2, Wen Yuan1, Lei Yang1
1Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
Abstract:
The role and miRNA expression profile of exosomes in hypertension remain largely unknown. Therefore, next-generation sequencing was used to define the miRNA expression profile of plasma exosomes in spontaneously hypertensive rats (SHRs), the most widely used animal model of human essential hypertension, and their controls, normotensive Wistar-Kyoto rats (WKYs). Results revealed that percentages of miRNA in the total small RNA isolated from SHRs and WKYs were not significantly different. Twenty-seven miRNAs were significantly differentially expressed (DE) between SHR and WKY exosomes, including 23 up-regulated and four down-regulated in SHR exosomes as compared to WKY exosomes. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis of top 10 DE miRNAs identified hypertension-specific target genes/signaling pathways. In conclusion, our findings indicated the selective packing of miRNA cargo into exosomes under hypertensive status, which could facilitate the development of potential targets for the diagnosis, prevention, and treatment of hypertension.
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Spontaneity
Overview of Exosomes
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Hypertension I: Introduction
Hypertension II: Pathophysiology
Hypertension V: Nursing Management

