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Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Ultraporous, Compressible, Wettable Polylactide/Polycaprolactone Sponges for Tissue Engineering
Michael Mader1, Valérie Jérôme2, Ruth Freitag2
1Macromolecular Chemistry and Bavarian Polymer Institute , University of Bayreuth , Universitätsstrasse 30 , 95440 Bayreuth , Germany.
Biomacromolecules
|March 22, 2018
Summary
Degradable ultraporous sponges from polylactide blends offer tunable properties for tissue engineering. These biocompatible scaffolds support rapid cell proliferation and high cell viability, showing great promise for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Science
Background:
- Developing advanced scaffolds is crucial for effective tissue regeneration.
- Biodegradable polymers offer promising platforms for creating biocompatible and resorbable tissue engineering constructs.
Purpose of the Study:
- To develop ultraporous, degradable sponges using polylactide (PLA) and PLA/poly(ε-caprolactone) (PCL) blends.
- To investigate the influence of processing parameters on sponge properties and assess their suitability for tissue engineering applications.
Main Methods:
- Freeze-drying of electrospun fiber dispersions followed by thermal annealing.
- Characterization of sponge porosity, cellular structure, mechanical properties (compressibility), and wettability.
- In vitro cell culture studies using Jurkat cells with Confocal Laser Scanning Microscopy, MTT, and Live/Dead staining.
Main Results:
- Ultrahigh porosity sponges (99.6%) with hierarchical cellular structures were fabricated.
- Sponges exhibited high reversible compressibility and rapid recovery in both dry and wet states.
- Tunable sponge properties (density, size, shape, mechanical strength, wettability) were achieved by adjusting fiber concentration and annealing temperature.
- Successful cell culture demonstrated rapid Jurkat cell proliferation and high cell viability on the sponges.
Conclusions:
- The developed polylactide-based sponges possess tunable, desirable characteristics for tissue engineering.
- These sponges demonstrate excellent biocompatibility and support cell growth, indicating significant potential for regenerative medicine.
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