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A cellular transcription factor E4F1 interacts with an E1a-inducible enhancer and mediates constitutive enhancer
The EMBO Journal
|May 1, 1987
Summary
Adenovirus E4 gene transcription relies on an E1a-dependent enhancer and a cellular factor, E4F1. E4F1 binding sites alone can drive transcription, suggesting a promoter model and involvement in co-regulated viral gene expression.
Area of Science:
- Molecular Biology
- Virology
- Gene Regulation
Background:
- Adenovirus E1a protein induces transcription of viral genes.
- The E4 gene's transcription is regulated by an enhancer element.
Purpose of the Study:
- To investigate the mechanism of adenovirus E4 gene transcription.
- To identify the role of cellular transcription factors in E4 gene regulation.
Main Methods:
- In vivo and in vitro transcription assays.
- Analysis of transcription factor binding sites within the E4 promoter region.
Main Results:
- An E1a-dependent enhancer controls E4 gene transcription in vivo.
- The transcription factor E4F1 binds to the E4 enhancer and a TATA box-proximal site.
- E4F1 binding sites alone can substitute for the enhancer, suggesting a promoter model.
- E4F1 interacts with other adenovirus early promoters.
Conclusions:
- The E4 promoter may consist of multiple E4F1 binding sites and a TATA box.
- E4F1 may coordinate the expression of E1a-inducible adenovirus early genes.