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Updated: Feb 13, 2026

Blood Flow Imaging with Ultrafast Doppler
Published on: October 14, 2020
Nonstaining Blood Flow Imaging Using Optical Interference Due to Doppler Shift and Near-Infrared Imaging of Molecular
Mika Ishigaki1, Paralee Puangchit1, Yui Yasui1
1School of Science and Technology , Kwansei Gakuin University , 2-1 Gakuen , Sanda , Hyogo 669-1337 , Japan.
Abstract:
In the present study, we successfully obtained nonstaining blood flow images of a developing fish egg embryo using optical interference caused by the Doppler shift. The spectral distribution of light reflected by moving objects such as the heart and red cells was found to be different from that of the incident light because of the Doppler effect. Interference between different frequency components was observed in an interferogram through heterodyne interaction using an imaging-type two-dimensional Fourier spectroscopic system, and information on the intensities of the spectral components was obtained by Fourier transformation. Beat signals with specific frequencies due to the heart beating and blood flow of the fish egg embryo were detected. When the signals were plotted in two dimensions, the heart part and vessel flows were clearly visualized without staining. In addition, near-infrared (NIR) images were produced using absorbance spectra of the molecular vibrations of O-H and C-H groups included in water, hydrocarbons, and aliphatic compounds. Obtaining nonstaining blood flow images using heterodyne optical interference and images of molecular distribution using molecular vibrational information simultaneously manifests an exciting advance in NIR imaging.
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