Related Experiment Video

Updated: Feb 13, 2026

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain
07:57

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

Published on: July 11, 2016

8.0K

Isolation and characterization of a cold-active, alkaline, detergent stable α-amylase from a novel bacterium Bacillus

Nihan Arabacı1, Burhan Arıkan1

  • 1a Department of Biology , Çukurova University , Adana , Turkey.

Preparative Biochemistry & Biotechnology
|March 22, 2018
PubMed

Keywords:
AlkalineBacillus subtiliscold-active α-amylasedetergentthin layer chromatography (TLC)zymogram

More Related Videos

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.7K
Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores08:58

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Published on: April 15, 2019

10.6K

Related Concept Videos

Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.9K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.5K
Cold Weather Concreting01:27

Cold Weather Concreting

When freshly poured concrete is exposed to freezing temperatures before it has set, the water within the concrete can freeze. This expansion disrupts the setting process, delays chemical reactions necessary for hardening, and increases the volume of pores within the hardened concrete, which weakens its overall structure. If the concrete manages to reach an appreciable strength before it freezes, the damage can be somewhat mitigated.
To counteract the negative impacts of cold weather, ensuring...
389
Masonry in Cold and Hot Weather Conditions01:21

Masonry in Cold and Hot Weather Conditions

In cold weather, masonry construction requires specific precautions to ensure mortar does not freeze before curing, as this can significantly weaken its strength and watertightness. Mortar temperature should be maintained between 60°F and 80°F to support proper hydration and curing. Below 40°F, mortar water must be heated, but should not exceed 120°F as high temperatures can reduce mortar's compressive and bond strength.
Other key practices include keeping masonry units...
351
α-Alkylation of Ketones via Enolate Ions01:10

α-Alkylation of Ketones via Enolate Ions

Ketones with α protons are deprotonated by strong bases like lithium diisopropylamide (LDA) to form enolate ions. The anion is stabilized by resonance, and its hybrid structure exhibits negative charges on the carbonyl oxygen and the α carbon. This ambident nucleophile can attack an electrophile via two possible sites: the carbonyl oxygen, known as O-attack, or the α carbon, known as C-attack. The nucleophilic attack via the carbanionic site is preferred. This is due to the...
3.9K
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
200.1K