Related Experiment Video
Updated: Feb 13, 2026

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
A silica sands-based method for faithful analysis of microbial communities and DNA isolation from a wide range of
Xia Liu1, Yongdong Xu1, Zhi Li1
1a Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology , Shenyang Agricultural University , Shenyang , China.
Abstract:
A silica sands-based method has been developed to isolate high quality genomic DNAs from cells of animals, plants and microorganisms, such as Hemisalanx prognathus, Spinacia oleracea, Pichia pastoris, Bacillus licheniformis and Escherichia coli. To the best of our knowledge, no DNA isolation method has so wide application until now. In addition, this method and a commercially available kit were compared in analysis of microbial communities using high-throughput 16s rDNA sequencing. As a result, the silica sands-based method was found to be even more efficient in isolating genomic DNA from gram-positive bacteria than the kit, indicating that it would become a very valuable choice to faithfully reflect the composition of microbial communities.
Related Concept Videos
What are Populations and Communities?
Keystone Species
Gene Regulation in Microbial Communities: Quorum Sensing
Community Based Intervention
Foundations of Community Mental Health Programs
Central to the success of community-based interventions is the...
DNA Isolation
DNA Isolation

