Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alterations in Respiration II01:30

Alterations in Respiration II

1.9K
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients.
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
1.9K
Altered States of Awareness01:06

Altered States of Awareness

1.2K
Altered states of consciousness represent significant deviations from one's normal mental state. These deviations can range from subtle changes in awareness to profound transformations in perception, thought processes, and sensory experiences. Altered states of consciousness can be triggered by various factors, including drug use, meditation, hypnosis, illness, or even intense fatigue.
The ingestion of substances like stimulants or hallucinogens leads to chemical alterations in the brain...
1.2K
What is Behavior?00:54

What is Behavior?

10.3K
Behaviors are actions that an organism engages in—they can be related to finding food, reproducing, defending against threats, and many other possible actions. Behaviors include activities related to the environment around the animal—such as migration—as well as social interactions within a species or population. Many behaviors involve motor output—that is, muscle movements—while others involve less visible actions, such as learning.
10.3K
Alterations in Blood Pressure01:30

Alterations in Blood Pressure

2.2K
Alterations in blood pressure, such as hypertension (high blood pressure) and hypotension (low blood pressure), significantly affect human health. Understanding these conditions' classifications, causes, and symptoms is essential for effective management and treatment.
Hypertension (High blood pressure)
Hypertension occurs when blood pressure readings consistently exceed the normal range. It is diagnosed when systolic blood pressure (the top number, indicating pressure while the heart...
2.2K
What are Cells?01:07

What are Cells?

204.9K
Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium or trillions of them, e.g., in humans. No matter what organism a cell is a part of, they share specific characteristics.
Basic Characteristics of Cells
A living cell has a plasma membrane, a bilayer of lipids that separates the aqueous solution inside the cell called the cytoplasm from the outside environment.
Furthermore, a living cell possesses genetic information...
204.9K
Cell Size01:22

Cell Size

132.0K
Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
132.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficiency of Four Irrigation Needles in Curved Simulated Root Canals.

Dentistry journal·2026
Same author

[Association between pericoronary fat attenuation index and high-risk plaques in patients with FFR-negative acute coronary syndrome].

Zhonghua xin xue guan bing za zhi·2026
Same author

Cross-Trial Interpretation of Programmed Death Ligand-1 Thresholds in Advanced Oesophageal Squamous Cell Carcinoma Requires Caution.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Does the Composite Endpoint Overstate Risk After Denosumab Discontinuation?

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Cyclic Fatigue of Rotary Versus Reciprocating Endodontic Files: An In Vitro Study of Engine-Driven Endodontic Files.

Dentistry journal·2026
Same author

High-Standard Non-Surgical Endodontic Treatment and Outcome: A Retrospective Follow-Up Study on Self-Assessment of Grading and Case Difficulty in an Academic Setting.

Dentistry journal·2025

Related Experiment Video

Updated: Feb 13, 2026

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
07:32

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Published on: September 25, 2021

3.6K

Human dental pulp stromal cell conditioned medium alters endothelial cell behavior.

M A Gharaei1, Y Xue1, K Mustafa1

  • 1Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Årstadveien 19, N-5009, Bergen, Norway.

Stem Cell Research & Therapy
|March 23, 2018
PubMed
Summary

Human dental pulp stromal cells (hDPSCs) secrete factors that promote blood vessel formation. Conditioned medium from hDPSCs significantly enhances endothelial cell attachment, proliferation, migration, and network formation in vitro.

More Related Videos

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.3K
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

2.8K

Related Experiment Videos

Last Updated: Feb 13, 2026

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
07:32

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Published on: September 25, 2021

3.6K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.3K
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

2.8K

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biomaterials Science

Background:

  • Angiogenesis is critical for tissue regeneration and repair.
  • Human dental pulp stromal cells (hDPSCs) exhibit angiogenic potential through paracrine signaling.
  • Understanding hDPSC-mediated endothelial cell (EC) guidance is crucial for regenerative therapies.

Purpose of the Study:

  • To investigate the effects of conditioned medium from hDPSCs (hDPSC-CM) on endothelial cell behavior in vitro.
  • To elucidate the paracrine mechanisms by which hDPSCs influence angiogenesis.

Main Methods:

  • hDPSCs were cultured, and their conditioned medium (hDPSC-CM) was analyzed for angiogenic factors using antibody arrays and ELISA.
  • Human umbilical vein endothelial cells (HUVECs) were treated with hDPSC-CM.
  • HUVEC proliferation, migration, attachment, and network formation (tubulogenesis) were assessed using various in vitro assays, including MTT, transwell migration, and Matrigel assays.

Main Results:

  • hDPSC-CM contained numerous proangiogenic factors, including vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1).
  • hDPSC-CM significantly enhanced HUVEC adhesion, proliferation, and migration compared to standard growth media.
  • Matrigel assays confirmed that hDPSC-CM promotes HUVEC network formation and tubulogenesis.

Conclusions:

  • hDPSC-CM exhibits potent proangiogenic and pro-maturation effects on endothelial cells.
  • The trophic factors secreted by hDPSCs effectively stimulate angiogenic responses, surpassing optimal culture conditions.
  • hDPSCs hold significant promise for applications in tissue regeneration and wound healing due to their angiogenic capabilities.