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Related Concept Videos

Stem Cell Niche01:26

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
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Role of Vitamins in Maintaining Bone Health01:25

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Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
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Embryonic Stem Cells00:58

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Related Experiment Video

Updated: Feb 13, 2026

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
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Stem cell niche-specific Ebf3 maintains the bone marrow cavity.

Masanari Seike1, Yoshiki Omatsu1, Hitomi Watanabe2

  • 1Laboratory of Stem Cell Biology and Developmental Immunology, Graduate School of Frontier Biosciences, Graduate School of Medicine, Immunology Frontier Research Center, World Premier International Research Center (WPI), Osaka University, Osaka 565-0871, Japan.

Genes & Development
|March 23, 2018
PubMed
Summary

Early B-cell factor 3 (Ebf3) is crucial for maintaining hematopoietic stem cell (HSC) niches. Its absence leads to impaired HSC function and osteosclerosis, highlighting Ebf3

Keywords:
Ebf3bonehematopoietic stem cellmesenchymal stem cellstem cell niche

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Bone Biology

Background:

  • Hematopoietic stem cells (HSCs) reside in specialized bone marrow niches.
  • Mesenchymal cells, including CXCL12-abundant reticular (CAR) or leptin receptor-positive (LepR+) cells, are key components of HSC niches.
  • The mechanism preventing osteogenesis in these cells to maintain marrow space is largely unknown.

Purpose of the Study:

  • To investigate the role of transcription factor early B-cell factor 3 (Ebf3) in maintaining HSC niches.
  • To understand how osteogenesis is regulated within the bone marrow microenvironment.

Main Methods:

  • Lineage tracing studies in mice.
  • Genetic deletion of Ebf3 and Ebf1 in CAR/LepR+ cells.
  • Analysis of HSC niche function and bone marrow morphology.

Main Results:

  • Ebf3 is preferentially expressed in self-renewing mesenchymal stem cells (CAR/LepR+ cells) within the bone marrow.
  • Deletion of Ebf3 impairs HSC niche function and causes osteosclerosis in aged mice.
  • Absence of Ebf1 and Ebf3 leads to osteosclerosis and marrow cavity occlusion, with CAR/LepR+ cells differentiating into osteoblasts.

Conclusions:

  • Ebf3 is essential for creating and maintaining functional HSC niches.
  • Ebf3 inhibits osteoblast differentiation of CAR/LepR+ cells, preserving space for HSCs.
  • Dysregulation of Ebf3 contributes to bone marrow pathologies like osteosclerosis.