Related Experiment Video
Updated: Feb 13, 2026

Foreign Accent and Forensic Speaker Identification in Voice Lineups: The Influence of Acoustic Features Based on Prosody
Published on: September 27, 2024
Influence of Biochemical Features of Burkholderia pseudomallei Strains on Identification Reliability by Vitek 2
Irina B Zakharova1, Yana A Lopasteyskaya1, Andrey V Toporkov1
1Department of Microbiology, Volgograd Plague Control Research Institute, Volgograd, 400131, Russian Federation.
Background:
Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium that causes melioidosis, a potentially fatal disease endemic in wet tropical areas. The currently available biochemical identification systems can misidentify some strains of B. pseudomallei. The aim of the present study was to identify the biochemical features of B. pseudomallei, which can affect its correct identification by Vitek 2 system.
Materials And Methods:
The biochemical patterns of 40 B. pseudomallei strains were obtained using Vitek 2 GN cards. The average contribution of biochemical tests in overall dissimilarities between correctly and incorrectly identified strains was assessed using nonmetric multidimensional scaling.
Results:
It was found (R statistic of 0.836, P = 0.001) that a combination of negative N-acetyl galactosaminidase, β-N-acetyl glucosaminidase, phosphatase, and positive D-cellobiase (dCEL), tyrosine arylamidase (TyrA), and L-proline arylamidase (ProA) tests leads to low discrimination of B. pseudomallei, whereas a set of positive dCEL and negative N-acetyl galactosaminidase, TyrA, and ProA determines the wrong identification of B. pseudomallei as Burkholderia cepacia complex.
Conclusion:
The further expansion of the Vitek 2 identification keys is needed for correct identification of atypical or regionally distributed biochemical profiles of B. pseudomallei.
Related Concept Videos
Reliability and Validity
Distribution Reliability and Automation
Toxidromes: Clinical Features
Thermal Strain
Shearing Strain
Measurements of Strain

