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Updated: Feb 13, 2026

Microfluidic Mixers for Studying Protein Folding
Published on: April 10, 2012
Acoustically enhanced microfluidic mixer to synthesize highly uniform nanodrugs without the addition of stabilizers
Nguyen Hoai An Le1, Hoang Van Phan1, Jiaqi Yu2
1Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, VIC.
Background:
This article presents an acoustically enhanced microfluidic mixer to generate highly uniform and ultra-fine nanoparticles, offering significant advantages over conventional liquid antisolvent techniques.
Methods:
The method employed a 3D microfluidic geometry whereby two different phases - solvent and antisolvent - were introduced at either side of a 1 μm thick resonating membrane, which contained a through-hole. The vibration of the membrane rapidly and efficiently mixed the two phases, at the location of the hole, leading to the formation of nanoparticles.
Results:
The versatility of the device was demonstrated by synthesizing budesonide (a common asthma drug) with a mean diameter of 135.7 nm and a polydispersity index of 0.044.
Conclusion:
The method offers a 40-fold reduction in the size of synthesized particles combined with a substantial improvement in uniformity, achieved without the need of stabilizers.
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