Related Experiment Video
Updated: Feb 13, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
Objective quantification of corneal haziness using anterior segment optical coherence tomography
Jeyanth Suresh Rose1, Juliet Eldrina1, Aarwin Joshua2
1Department of Ophthalmology, Christian Medical College, Vellore, India.
Purpose:
To quantify normal corneal transparency by anterior segment optical coherence tomography (AS-OCT) by measuring the average pixel intensity. To analyze the variation in the average pixel intensity in mild and severe grades of corneal opacities.
Methods:
This is an observational, cross-sectional study of 38 eyes from 19 patients with mild or severe grades of corneal opacities greater than 3 mm and a normal contralateral cornea. AS-OCT was performed centered on the opacity with a 3 mm cruciate protocol. A similar image is taken of the contralateral clear cornea in the same quadrant. The average pixel intensity was calculated in a standardized manner using MATLAB software.
Result:
The average pixel intensity of the normal cornea was 99.6 ± 10.9 [standard deviation (SD)]. The average pixel intensity of the mild and severe corneal opacities was 115.5 ± 9.1 and 141.1 ± 10.3, respectively. The differences were statistically significant.
Conclusions:
AS-OCT images can be used to quantify corneal transparency. Average pixel intensity is a measure that varies significantly with varying corneal opacification.
Related Concept Videos
Velocity of an Object
Muscles of the Anterior Neck
Potential Due to a Polarized Object
Potential Due to a Magnetized Object
The vector...
Moment of Inertia of Compound Objects
Consider a child of mass (mc) 25 kg standing at a distance (rc) of 1 m from the axis of a rotating...
Computed Tomography
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

