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Updated: Feb 13, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
pH-Responsive spherical nucleic acid for intracellular lysosome imaging and an effective drug delivery system
Hui Li1, Xiang Zhou2, Dongbao Yao2
1CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China. hjliang@ustc.edu.cn and Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Abstract:
Herein, a class of pH-responsive spherical nucleic acid (SNA) nanostructures prepared by functionalizing gold nanoparticles with DNA nanodevices is presented, which can perform duplex-to-triplex transition upon pH change via pH-sensitive parallel Hoogsteen interactions. The SNA nanostructures exhibit tunable pH sensitivity and a highly rapid and reversible allosteric effect. Furthermore, they can exactly image lysosomes in living cells. We next engineered pH-triggered doxorubicin(Dox)-SNA conjugates that could deliver Dox molecules into cancer cells and achieve pH-dependent drug release.
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