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Related Concept Videos

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Introduction to Fibroblasts

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Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
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Cardiac output (CO), the amount of blood the heart pumps per minute, is a parameter in cardiovascular physiology determined by stroke volume and heart rate. Stroke volume, the amount of blood pushed from one of the ventricles per heartbeat, is influenced by preload, afterload, and contractility.
Preload
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The cardiac cycle refers to the sequence of events that occur in the heart from the beginning of one heartbeat to the next. It's characterized by alternating periods of contraction (systole) and relaxation (diastole) of the heart muscles.
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Cardiac action potentials are essential for proper heart function, enabling the rhythmic contractions needed for adequate blood circulation. Nodal cells and Purkinje fibers, specialized for electrical conduction, generate these action potentials.
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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
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Embryonary Mouse Cardiac Fibroblast Isolation.

Alejandra Garate-Carrillo1, Israel Ramirez2

  • 1Department of Medicine, School of Medicine, University of California, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 23, 2018
PubMed
Summary

This study presents a reliable method for culturing primary embryonic mouse cardiac fibroblasts. This technique ensures high cell yield and viability, crucial for studying heart disease mechanisms and potential therapies.

Keywords:
Cardiac fibroblastsCell cultureEmbryonic miceEnzymatic digestionHeartIsolation

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Area of Science:

  • Cardiovascular Biology
  • Cell Culture Techniques
  • Developmental Biology

Background:

  • Mouse cardiac fibroblasts (FBs) are vital in vitro models for cardiac research.
  • Primary FBs exhibit rapid senescence and phenotypic changes in culture post-birth.
  • Embryonic fibroblast cultures offer a more stable and desirable model.

Purpose of the Study:

  • To describe a method for primary culture of embryonic mouse cardiac fibroblasts.
  • To achieve good cell yield and viability in these cultures.
  • To provide a reliable model for cardiac pathology research.

Main Methods:

  • Utilizing E16 CD-1 mouse embryos.
  • Establishing primary cell cultures from embryonic cardiac tissue.
  • Optimizing culture conditions for fibroblast yield and viability.

Main Results:

  • Successful primary culture of E16 CD-1 mouse embryonic cardiac fibroblasts.
  • Demonstrated good cell yield from the described method.
  • Achieved high cell viability in the primary cultures.

Conclusions:

  • The described method provides a robust approach for obtaining viable embryonic mouse cardiac fibroblasts.
  • This technique supports consistent research into cardiac diseases and therapeutic targets.
  • Primary embryonic cardiac fibroblast cultures are superior for long-term in vitro studies.