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Published on: June 19, 2019
Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APC) Populations
Ju Hee Lee1,2, Azadeh Yeganeh1, Hisato Konoeda1
1Translational Medicine, The Hospital for Sick Children, Toronto, ON, Canada.
Abstract:
Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immunophenotyping, quantification, and isolation of distinct populations, which is critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of adipocyte precursor cells using flow cytometry and FACS, defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the plasticity and the cellular origin of APCs.
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