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Updated: Feb 12, 2026

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
Published on: April 17, 2011
Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice
Seul Ki Lee1, Tiffany M Schmidt2
1Department of Neurobiology, Northwestern University, Evanston, IL, USA.
Abstract:
Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and physiological properties. ipRGC subtypes can be identified morphologically based on a combination of dendritic morphology and immunostaining for a cell-type specific marker. In this chapter, we describe methods for conclusively identifying each of the five ipRGC subtypes through a combination of patch clamp electrophysiology, Neurobiotin filling, visualization of ipRGC dendrites, and immunostaining for the marker SMI-32.
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