Host Respiration Rate and Injury-Derived Cues Drive Host Preference by an Ectoparasite of Fruit Flies

Host bioenergetics and energy fluxes can be applied to measure the ecological and physiological effects of parasitism. By measuring changes in host metabolic rate, one can estimate the physiological costs of infection. Additionally, metabolic rate dictates the rate of resource conversion within a host and, by extension, the resources available to a parasite. We hypothesize that parasites are selected to respond to cues that indicate high resource availability, that is, host metabolic state. We investigated whether an ectoparasite mite (Macrocheles subbadius) can differentiate between potential hosts (Drosophilia nigrospiracula) on the basis of relative carbon dioxide output as measured by respirometry. In pairwise choice tests, mites were allowed to choose between two size-matched fruit flies with differing metabolic rates or levels of CO₂ output. Our results showed that mites preferentially infect flies with relatively higher respiration rates. Accordingly, we investigated whether fly respiratory rate (measured by CO₂ production) changes in response to injury, potentially explaining a previously reported preference for injured flies. We also tested whether chemical cues released during injury influence preference for injured hosts. We determined that fly exudate (mostly consisting of hemolymph) applied to an uninjured fly released at the site of injury significantly increased the likelihood of infection, but injury did not significantly change the CO₂ output of the flies. Our results suggest that parasites are relying on chemical cues not only for host finding but also to discriminate between hosts on the basis of the rate of respiration, with potentially important implications for the metabolic theory of ecology.