Related Experiment Video
Updated: Feb 12, 2026
10:05
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
10.1K
Association of Proteomics Changes with Al-Sensitive Root Zones in Switchgrass
Mahesh Rangu1, Zhujia Ye2, Sarabjit Bhatti3
1Department of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, 3500 John Merritt Blvd, Nashville, TN 37209, USA. mahesh.rng@gmail.com.
Proteomes
|March 23, 2018
Summary
Aluminum (Al) stress significantly alters switchgrass root proteomes, particularly in the apical tip. This study identifies key Al-responsive proteins, offering insights into plant tolerance mechanisms.
Area of Science:
- Plant Biology
- Proteomics
- Environmental Stress Response
Background:
- Aluminum (Al) toxicity is a major abiotic stress limiting plant growth in acidic soils.
- Switchgrass (Panicum virgatum) is a vital bioenergy crop susceptible to Al toxicity.
- Understanding Al-induced molecular changes in roots is crucial for developing tolerant cultivars.
Purpose of the Study:
- To investigate the proteomic alterations in switchgrass roots in response to aluminum (Al) exposure.
- To identify specific proteins and biological pathways affected by Al stress in different root zones.
Main Methods:
- Hydroponic culture system with 400 μM Al treatment.
- Quantitative proteomics using tandem mass tags mass spectrometry (TMT-MS).
- Analysis of protein changes in apical (0-1 cm) and elongation/maturation (1-2 cm) root zones.
Main Results:
- A total of 216 proteins exhibited significant changes under Al stress (FDR adjusted p-value < 0.05).
- Apical root tips showed more pronounced proteomic changes (164 proteins) than elongation/maturation zones (52 proteins).
- Al-induced proteins were involved in cell cycle regulation (apical) and secondary metabolism, specifically lignin biosynthesis (elongation/maturation zones).
Conclusions:
- Aluminum stress profoundly impacts switchgrass root proteomes, with distinct responses in different root regions.
- Identified Al-responsive proteins, including transcription factors, provide targets for enhancing Al tolerance in switchgrass.
- This proteomic dataset offers valuable resources for future research into plant Al tolerance mechanisms.
Related Concept Videos
02:29Hybrid Zones
22.0K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
22.0K
03:02Primary and Secondary Growth in Roots and Shoots
60.7K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.7K
01:16Zones of Protection
815
In power systems, the entire setup is divided into protective zones to isolate faults and protect the rest of the network. These zones include generators, transformers, buses, transmission lines, distribution lines, and motors. Each zone can be visualized as a separate room in a house, with each room protected by its own circuit breaker.
Protective zones are defined by closed dashed lines, containing one or more components. A key characteristic of these zones is the strategic placement of...
Protective zones are defined by closed dashed lines, containing one or more components. A key characteristic of these zones is the strategic placement of...
815
01:28Transition Zone
396
The transition zone in concrete is a critical area where aggregate meets cement paste, marked by a distinct porosity and weakness compared to the surrounding material. The adhesion around the aggregates is primarily due to Van Der Waals forces. The voids within this zone influence its robustness; initially, it is less durable than the surrounding bulk mortar due to larger voids. Initially, when concrete is compacted, a higher water-cement ratio near the aggregates leads to the formation of...
396
01:33Proteomics
9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K
01:27Associative Learning
1.5K
Associative learning is a fundamental concept in behavioral psychology, wherein a connection is established between two stimuli or events, leading to a learned response. This process is critical in understanding how behaviors are acquired and modified. Conditioning, the mechanism through which associations are formed, can be divided into two main types: classical conditioning and operant conditioning, each elucidating different aspects of associative learning.
Classical conditioning, also known...
Classical conditioning, also known...
1.5K