Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-gated Ion Channels01:24

Non-gated Ion Channels

8.3K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
8.3K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

5.8K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
5.8K
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

14.4K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
14.4K
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

11.0K
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
11.0K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

7.8K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
7.8K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.1K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HCN1 is a primary HCN Pacemaker Channel in Neurons.

Nature communications·2026
Same author

Using Bayesian priors to overcome non-identifiablility issues in Hidden Markov models.

bioRxiv : the preprint server for biology·2025
Same author

Functional control of heteromeric Kv2.1/6.4 channels by the voltage sensor domain of the silent Kv6.4 subunit.

The Journal of physiology·2025
Same author

Leveraging electronic medical records to evaluate a computerized decision support system for staphylococcus bacteremia.

NPJ digital medicine·2025
Same author

Subunit-specific conductance of single homomeric and heteromeric HCN pacemaker channels at femtosiemens resolution.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Subtype-Specific Ligand Binding and Activation Gating in Homomeric and Heteromeric P2X Receptors.

Biomolecules·2024
Same journal

Another 10 years of PLOS Computational Biology: A data-driven reflection on trends in genomics research.

PLoS computational biology·2026
Same journal

Mobility data resolution needed to inform predictive models of spatial epidemic spread from mobile phone data.

PLoS computational biology·2026
Same journal

DeepMethylation: A deep learning framework for tissue-specific DNA methylation prediction and functional variant annotation.

PLoS computational biology·2026
Same journal

Redefining and estimating the early-phase reproduction ratio for epidemic outbreaks in spatially structured populations.

PLoS computational biology·2026
Same journal

Optimized phenotype definitions boost GWAS power.

PLoS computational biology·2026
Same journal

Detection, communication, and individual identification with deep audio embeddings: A case study with North Atlantic right whales.

PLoS computational biology·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

11.1K

Activation gating in HCN2 channels.

Sabine Hummert1, Susanne Thon1, Thomas Eick1

  • 1Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, Jena, Germany.

Plos Computational Biology
|March 23, 2018
PubMed
Summary
This summary is machine-generated.

Hyperpolarization-activated cyclic nucleotide-modulated (HCN) channels control electrical rhythmicity. cAMP binding stabilizes HCN2 channel opening, alters gating charge, and accelerates activation, revealing detailed insights into channel gating mechanisms.

More Related Videos

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

33.5K
Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
07:31

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

Published on: September 27, 2011

15.6K

Related Experiment Videos

Last Updated: Feb 12, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

11.1K
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

33.5K
Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
07:31

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

Published on: September 27, 2011

15.6K

Area of Science:

  • Molecular biology
  • Biophysics
  • Neuroscience

Background:

  • Hyperpolarization-activated cyclic nucleotide-modulated (HCN) channels are crucial for electrical rhythmicity in excitable cells.
  • Understanding the precise mechanisms of HCN channel gating is essential for comprehending cellular electrophysiology.

Purpose of the Study:

  • To quantitatively analyze the voltage-dependent activation of homotetrameric HCN2 channels.
  • To investigate the modulatory effects of cyclic AMP (cAMP) on HCN2 channel gating.

Main Methods:

  • Global fitting of hidden Markovian models to experimental data.
  • Quantitative analysis of voltage-dependent activation and cAMP modulation.

Main Results:

  • HCN2 channel activation involves two voltage-dependent steps followed by voltage-independent pore opening.
  • cAMP binding stabilizes the open state, reduces gating charge, and accelerates ON-gating.
  • The open HCN2 channel exhibits slower OFF-gating current compared to the closed state.

Conclusions:

  • The study provides a detailed mechanistic model for voltage- and cAMP-induced gating of HCN channels.
  • These findings enhance our understanding of the electrophysiological roles of HCN channels in the brain and heart.