Related Experiment Video

Updated: Feb 12, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

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Oleanolic acid induces osteosarcoma cell apoptosis by inhibition of Notch signaling

Ying Xu1, Bing Shu2, Ye Tian3

  • 1Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, P.R. China.

Molecular Carcinogenesis
|March 23, 2018
PubMed

Insights

Oleanolic acid (OA) effectively inhibits osteosarcoma cell growth and survival. It triggers programmed cell death (apoptosis) via the mitochondrial pathway, independent of Notch signaling, suggesting potential as an adjuvant cancer therapy.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Oleanolic acid (OA), a natural triterpenoid, shows promise as an anticancer agent.
  • The precise mechanisms of OA's effects on osteosarcoma cell death require further investigation.

Purpose of the Study:

  • To elucidate the mechanism of OA-induced apoptosis in osteosarcoma cells.
  • To investigate the role of Notch signaling in OA's antitumor effects.

Main Methods:

  • Dose-dependent proliferation and viability assays.
  • Flow cytometry to detect apoptosis.
  • Western blot and mitochondrial membrane potential assays.
  • Investigation of Notch signaling pathway activation.

Main Results:

  • OA significantly inhibited osteosarcoma cell proliferation and viability in a dose-dependent manner.
  • OA treatment induced significant apoptosis, which was blocked by Jagged1-mediated Notch signaling.
  • OA's mechanism involves the mitochondrial apoptosis pathway and disrupts the balance of apoptotic/anti-apoptotic factors.
  • OA induces osteosarcoma cell apoptosis by targeting mitochondria in a Notch signaling-dependent manner.

Conclusions:

  • Oleanolic acid induces osteosarcoma cell apoptosis through the mitochondrial pathway.
  • The Notch signaling pathway plays a role in mediating OA's effects on osteosarcoma cells.
  • OA demonstrates potential as an adjuvant therapeutic agent for osteosarcoma.
Keywords:
Notch signalingcell apoptosisoleanolic acidosteosarcoma

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