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Related Concept Videos

Tooth Anatomy01:21

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Related Experiment Video

Updated: Feb 12, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
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Wnt-Responsive Odontoblasts Secrete New Dentin after Superficial Tooth Injury.

Y Zhao1,2, X Yuan2, B Liu3

  • 11 Department of Oral Basic Science, School of Dentistry, Lanzhou University, Lanzhou, China.

Journal of Dental Research
|March 23, 2018
PubMed
Summary
This summary is machine-generated.

New therapeutic strategies for stimulating dentin formation involve targeting Wnt signaling. In adult teeth, superficial injury activates Wnt-responsive odontoblasts, promoting new dentin deposition and enhancing pulp repair.

Keywords:
Wnt3A proteincell deathdental pulpdentinogenesismolarregeneration

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Dental Research

Background:

  • Dentin formation is crucial for tooth repair after injury.
  • Current therapeutic strategies for pulp capping have limitations in stimulating natural dentin regeneration.
  • Understanding the cellular and molecular responses to dental injury is key to developing novel treatments.

Purpose of the Study:

  • To identify new therapeutic strategies for stimulating dentin formation in adult teeth.
  • To evaluate dentin production in response to acute pulp exposure and superficial dentin injury.
  • To investigate the role of Wnt signaling in dentin repair.

Main Methods:

  • Utilized two acute trauma models: pulp exposure and superficial dentin injury.
  • Performed molecular, cellular, and histologic analyses to assess repair responses.
  • Employed a tamoxifen-inducible reporter strain (Axin2CreERT2/+; R26RmTmG/+) to track odontoblast activity.
  • Investigated the effect of a liposomal formulation of human WNT3A protein on Wnt signaling.

Main Results:

  • Severe pulp exposure led to widespread cell death, with repair initiated by surviving pulp cells and odontoblasts.
  • Superficial dentin injury preserved pulp vitality and innervation, with primary odontoblasts upregulating HIF1α and increasing mineralization.
  • Wnt-responsive, long-lived odontoblasts were identified as responsible for new dentin deposition after superficial injury.
  • Liposomal WNT3A delivered via dentinal tubules effectively upregulated Wnt signaling in the pulp.

Conclusions:

  • Wnt signaling amplification in the pulp stimulates dentin secretion.
  • A liposomal WNT3A formulation shows promise as a therapeutic pulp-capping agent.
  • These findings provide a strong proof of concept for enhancing pulp repair through targeted Wnt signaling stimulation.