Effect of graphene oxide on the bioactivities of nitrifying and denitrifying bacteria in aerobic granular sludge

With the widespread application of graphene oxide (GO), it would be inevitably released into wastewater treatment plants (WWTPs) and get involved in the biochemical process. So far, there are controversies on the effects of low GO concentration (0.05-0.1 g/L) on the nitrogen removal process. Therefore, this study essentially investigates any potential effects of GO on wastewater microbial communities functions. In present study, the nitrifying and denitrifying batch tests were introduced to investigate the influence of 0.06 g/L of GO on bacteria. The results showed that GO could be easily combined with the aerobic granular sludge (AGS), and NH₄⁺-N was sharply absorbed, which directly promoted the bioactivities of ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) and extracellular polymeric substances (EPS) production. The influence of GO on the denitrifying bacteria was negligible, which resulted in the stable EPS production. Furthermore, as inferred from the near maximum chemical reaction rates, there were no obvious changes on the microbial community functions during nitrogen removal process.