Effect of gestational exposure to arsenic on puberty in offspring female mice

We examined the vaginal opening day, ovary and uterus organ coefficient, reproductive hormone levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH), mRNA and protein expression levels of kiss-1, hypothalamus gonadotrophin releasing hormone 1 (GnRH1), organic cation transporters 2 (Oct2) and transcription termination factor 1 (Ttf1) in different pubertal ages [late lactation (18 days), pre-puberty (21-22 days), puberty (23-27 days; with respect to vaginal opening) and maturity (65 days)] of offspring females, to evaluate the effect of arsenic (As) on puberty initiation after maternal exposure to As at different concentration [0, 0.15, 1.5 and 15 mg/L As(III)] during gestational period. The results showed that the vaginal opening time was significantly advanced in utero in mice exposed to As compared to the control. The hormone level of LH was significantly increased in the mice treated with 15 mg/L of As(III) at puberty compared to the control. During puberty, the mRNA expression levels of kiss-1, GnRH1, Oct2 and Ttf1 in the hypothalamus were significantly increased in the group treated with 15 mg/L of As(III) compared to the control. The protein expressions of Kisspeptin, GnRH1, Oct2 and Ttf1 in the hypothalamus were significantly increased in the pubertal females, while Oct2 and Ttf1 expression levels were significantly decreased in the matured females compared to the control, which is in line with the transcriptional changes of related mRNA expressions. In brief, this study demonstrated that maternal exposure to As during gestational period could result in early onset of puberty in offspring females.