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Updated: Feb 12, 2026

Western Blotting: Sample Preparation to Detection
Published on: October 14, 2010
Validation of a yeast malate dehydrogenase 2 (Mdh2) antibody tested for use in western blots
Shiran Gabay-Maskit1, Maya Schuldiner1, Einat Zalckvar1
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
Abstract:
Malate dehydrogenases (Mdhs) reversibly convert malate to oxaloacetate and serve as important enzymes in several metabolic pathways. In the yeast Saccharomyces cerevisiae there are three Mdh isozymes, localized to different compartments in the cell. In order to identify specifically the Mdh2 isozyme, GenScript USA produced three different antibodies that we further tested by western blot. All three antibodies recognized the S. cerevisiae Mdh2 with different background and specificity properties. One of the antibodies had a relatively low background and high specificity and thus can be used for specific identification of Mdh2 in various experimental settings.
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