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Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In

Shumei Wang1, Xue Han1,2, Lanwei Zhang1,3

  • 1Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin 150030, China.

Biomed Research International
|March 24, 2018
PubMed

Insights

Whole peptidoglycan (WPG) from Lactobacillus paracasei M5 shows anticancer potential. This WPG induces apoptosis in colon cancer cells, offering a promising natural compound for cancer therapy.

Area of Science:

  • Microbiology
  • Biochemistry
  • Cancer Biology

Background:

  • Lactobacillus paracasei M5 exhibits potential anticancer properties.
  • The cell wall components, specifically whole peptidoglycan (WPG), are investigated for their biological activities.

Purpose of the Study:

  • To extract and characterize WPG from Lactobacillus paracasei M5.
  • To evaluate the anticancer effects of WPG on HT-29 colon cancer cells.
  • To elucidate the mechanism of WPG-induced cell death.

Main Methods:

  • Whole peptidoglycan (WPG) extraction and SDS-PAGE analysis.
  • Amino acid composition analysis.
  • Cell viability assays (MTT, trypan blue exclusion).
  • Apoptosis analysis (transmission electron microscopy, flow cytometry, RT-PCR).

Main Results:

  • WPG was successfully extracted and characterized, showing a dominant band at approximately 14.4 kDa.
  • WPG demonstrated dose-dependent cytotoxic effects on HT-29 colon cancer cells.
  • WPG upregulated proapoptotic genes and downregulated antiapoptotic genes, inducing a mitochondria-mediated apoptotic pathway.

Conclusions:

  • WPG from Lactobacillus paracasei M5 possesses significant anticancer properties against colon cancer cells.
  • WPG induces apoptosis through the mitochondria-mediated pathway.
  • This study highlights WPG as a potential therapeutic agent in cancer treatment.

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