Related Experiment Video
Updated: Feb 12, 2026
12:32
Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
Published on: March 13, 2018
7.0K
Anisakis pegreffii impacts differentiation and function of human dendritic cells
C Napoletano1, S Mattiucci2, A Colantoni2
1Department of Experimental Medicine, "Sapienza" University of Rome, Rome, Italy.
Parasite Immunology
|March 24, 2018
Summary
Anisakis pegreffii larvae impact human dendritic cell (DC) function, reducing their ability to present antigens and activate T-cells. This study reveals the parasite
Area of Science:
- Immunology
- Parasitology
- Cell Biology
Background:
- Helminth infections often induce immune tolerance through dendritic cell (DC) modulation.
- Anisakis pegreffii is a zoonotic nematode with potential immunomodulatory effects.
Purpose of the Study:
- To investigate the interaction between Anisakis pegreffii and human monocyte-derived dendritic cells (DCs).
- To understand how A. pegreffii influences DC maturation, function, and cytokine secretion.
Main Methods:
- Co-culture of human monocyte-derived DCs with live Anisakis pegreffii larvae or crude extracts.
- Analysis of DC surface marker expression (HLA-DR, CD86, CD83, CCR7).
- Measurement of phagosomal reactive oxygen species (ROS) and ERK1,2 pathway phosphorylation.
- Assessment of DC-mediated T-cell activation (IFNγ production) and cytokine/chemokine secretion.
Main Results:
- A. pegreffii impacted DC viability and maturation, reducing key antigen presentation and migration molecules.
- Phagosomal ROS levels increased, and the ERK1,2 pathway was modulated.
- DCs exposed to A. pegreffii showed impaired ability to activate T-cell-mediated IFNγ.
- Live larvae and crude extracts differentially modulated DC cytokine and chemokine secretion.
Conclusions:
- Anisakis pegreffii exerts significant immunomodulatory effects on dendritic cell biology and function.
- DCs play a dynamic role in the inflammatory response against A. pegreffii infections.
- These findings shed light on the host-parasite immune interactions in anisakiasis.
Related Concept Videos
01:23Neurons: The Cell Body and the Dendrites
7.3K
A typical nerve cell comprises three main components: the cell body, dendrites, and the axon. The cell body, also known as the soma or perikaryon, serves as the central biosynthetic hub housing a nucleus surrounded by cytoplasm containing organelles commonly found in most cells. Notably, Nissl bodies, clusters of the rough endoplasmic reticulum and free ribosomes responsible for protein synthesis, are distinctive features of the neuronal cell body. As neurons age, aggregates of a brown pigment...
7.3K
01:19Impact of Groups on Groups
262
Social psychologists analyze how groups influence one another, shaping social structures and interactions through both cooperation and competition. These dynamics manifest in various ways, ranging from economic partnerships to intergroup conflicts that shape societal structures and perceptions.Cooperation and Competition in Intergroup RelationsIntergroup relationships vary across contexts, sometimes fostering cooperation and mutual benefit while at other times leading to conflict and...
262
01:22iPS Cell Differentiation
3.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.2K
01:24B Cell Activation and Differentiation
16.9K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
16.9K
01:30Impact
513
Impact occurs when two bodies collide, leading to the application of impulsive forces between them. Analyzing impact mechanics involves considering two colliding particles moving along a line known as the line of impact, which passes through their centers and is perpendicular to the contact plane.
When particles with different initial velocities collide, they induce deformation by applying equal and opposite impulses. At the point of maximum deformation, the particles move together with...
When particles with different initial velocities collide, they induce deformation by applying equal and opposite impulses. At the point of maximum deformation, the particles move together with...
513
01:25Compounds Essential to Human Function
10.5K
The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
10.5K