Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Networks02:26

Protein Networks

2.9K
2.9K
Compounds Essential to Human Function01:25

Compounds Essential to Human Function

10.5K
The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
10.5K
Essential Minerals for Bone Health01:31

Essential Minerals for Bone Health

6.6K
The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
6.6K
Subcellular Fractionation01:32

Subcellular Fractionation

8.9K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.9K
Extraction: Partition and Distribution Coefficients01:14

Extraction: Partition and Distribution Coefficients

4.9K
The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
For extracting a solute from an aqueous phase into an...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IIC-DTI: A Contrastive Learning Enhanced Inter-Intra Molecular Fusing Framework for Drug-Target Interaction Prediction.

Interdisciplinary sciences, computational life sciences·2026
Same author

Graph convolution network based on meta-paths and mutual information for drug-target interaction prediction.

BMC bioinformatics·2025
Same author

The study of the variation of mineral distribution and relative concentration on varieties of oat using synchrotron-based X-ray fluorescence imaging.

Food research international (Ottawa, Ont.)·2025
Same author

Predicting miRNA-Drug Interactions Based on Multi-source Feature Fusion of Heterogeneous Network.

Interdisciplinary sciences, computational life sciences·2025
Same author

Low-Count PET Image Reconstruction With Generalized Sparsity Priors via Unrolled Deep Networks.

IEEE journal of biomedical and health informatics·2025
Same author

Unraveling bladder cancer-related circRNA biomarkers: a hybrid model combining deep learning and statistics.

Health information science and systems·2025

Related Experiment Video

Updated: Feb 12, 2026

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

1.3K

Identifying essential proteins based on sub-network partition and prioritization by integrating subcellular

Min Li1, Wenkai Li1, Fang-Xiang Wu2

  • 1School of Information Science and Engineering, Central South University, Changsha 410083, China.

Journal of Theoretical Biology
|March 25, 2018
PubMed
Summary

Identifying essential proteins is crucial for understanding diseases and developing drugs. A new method, SPP, uses sub-network partitioning and subcellular localization to improve essential protein prediction accuracy in protein-protein interaction networks.

Keywords:
Biological informationEssential proteinNetwork topologyProtein-protein interaction network

More Related Videos

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
11:11

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Published on: June 15, 2018

8.8K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

9.4K

Related Experiment Videos

Last Updated: Feb 12, 2026

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

1.3K
Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
11:11

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Published on: June 15, 2018

8.8K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

9.4K

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Systems Biology

Background:

  • Essential proteins are vital for organism survival and reproduction.
  • Protein-protein interaction (PPI) networks are key to studying complex diseases and drug design.
  • Existing methods for identifying essential proteins from PPI networks are often hindered by data noise.

Purpose of the Study:

  • To develop a novel network-based method for accurate essential protein identification.
  • To integrate subcellular localization information to overcome limitations of existing PPI network analysis.
  • To improve the prediction accuracy of essential proteins by reducing the impact of false positives.

Main Methods:

  • A new method, SPP (sub-network partition and prioritization), was developed.
  • SPP integrates subcellular localization information with network topology analysis.
  • The method was validated on yeast PPI networks from DIP and BioGRID databases.

Main Results:

  • SPP effectively reduces the impact of false positives in PPI data.
  • The proposed method demonstrates higher accuracy in predicting essential proteins compared to existing computational approaches (DC, BC, CC, SC, EC, IC, NC).
  • Experimental results confirm the efficacy of SPP in enhancing essential protein discovery.

Conclusions:

  • SPP offers a more accurate and robust approach for identifying essential proteins.
  • Integrating subcellular localization information is a valuable strategy for improving PPI network analysis.
  • The method has significant implications for disease research, drug discovery, and bioinformatics.