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Long noncoding RNA BDNF-AS is downregulated in cervical cancer and has anti-cancer functions by negatively

Huimin Zhang1, Caihong Liu1, Ting Yan1

  • 1Department of Gynaecology, Guizhou Provincal People's Hospital, Guiyang, Guizhou Province, 550002, China.

Archives of Biochemistry and Biophysics
|March 25, 2018
PubMed
Abstract

Insights

Long noncoding RNA BDNF-AS is downregulated in cervical cancer (CC). Overexpressing BDNF-AS inhibits CC cell proliferation and migration, potentially via inverse regulation of BDNF.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cervical cancer (CC) remains a significant global health challenge.
  • Understanding the molecular mechanisms underlying CC progression is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To investigate the expression and functional role of the long noncoding RNA (lncRNA) BDNF-AS in human cervical cancer.
  • To elucidate the mechanism by which BDNF-AS influences CC progression, particularly its relationship with BDNF.

Main Methods:

  • Quantitative real-time PCR (qPCR) was used to assess BDNF-AS and BDNF expression in CC cell lines and tumor tissues.
  • Lentiviral vectors were employed to overexpress BDNF-AS in CC cells (SiHa and DoTc2-4510).
  • In vitro assays evaluated the effects of BDNF-AS overexpression on CC cell proliferation and migration, while in vivo studies assessed tumor transplantation.

Main Results:

  • BDNF expression was found to be downregulated in both CC cell lines and human CC tumors.
  • Overexpression of BDNF-AS demonstrated anti-cancer effects by inhibiting CC cell proliferation and migration in vitro, and reducing tumor growth in vivo.
  • BDNF-AS and BDNF exhibited inverse expression patterns in CC.
  • Restoring BDNF levels in BDNF-AS-overexpressing cells reversed the observed anti-cancer effects.

Conclusions:

  • BDNF-AS is significantly downregulated in cervical cancer.
  • Ectopic expression of BDNF-AS exhibits potent anti-cancer properties in CC, likely through the inverse regulation of BDNF.
  • Targeting BDNF-AS may represent a promising therapeutic avenue for cervical cancer treatment.
Keywords:
BDNFBDNF-ASCervical cancerMigrationProliferationTransplantation

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