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MiR-127-3p inhibits cell growth and invasiveness by targeting ITGA6 in human osteosarcoma

Dong Wang1, Liang Tang1, Huihui Wu2,3

  • 1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

IUBMB Life
|March 25, 2018
PubMed

Insights

MicroRNA-127-3p (miR-127-3p) suppresses osteosarcoma (OS) progression by inhibiting cell proliferation, invasion, and migration while promoting apoptosis. It achieves this by targeting integrin subunit-α 6 (ITGA6), which is upregulated in OS.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a prevalent pediatric bone cancer.
  • Understanding the molecular mechanisms driving OS is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the roles of miR-127-3p and integrin subunit-α 6 (ITGA6) in OS.
  • To elucidate the molecular mechanism and target relationship between miR-127-3p and ITGA6 in OS.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot to assess miR-127-3p and ITGA6 expression.
  • Dual-luciferase reporter assay to confirm the target relationship.
  • Cell counting kit 8 (CCK-8), colony formation, flow cytometry, caspase 3 activity, transwell, and wound healing assays to evaluate biological functions.

Main Results:

  • miR-127-3p was significantly downregulated, while ITGA6 was upregulated in OS tissues and cells.
  • ITGA6 was identified as a direct downstream target of miR-127-3p.
  • miR-127-3p suppressed OS cell proliferation, invasion, and migration, and promoted apoptosis, partly by downregulating ITGA6.

Conclusions:

  • miR-127-3p acts as a tumor suppressor in OS.
  • ITGA6 functions as an oncogene in OS.
  • Targeting the miR-127-3p/ITGA6 axis may offer a therapeutic strategy for osteosarcoma.
Keywords:
ITGA6miR-127-3posteosarcoma

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