Cytotoxic function of a cloned helper T cell line

The cytotoxic function of a well-characterized helper T hybridoma was investigated utilizing 2,4,6-trinitrophenyl (Tnp) specific B cells. The T hybridoma which is ovalbumin (Ova) specific recognizes the Ova synthetic peptide 323-339 Tyr. A number of experiments using several different antigens, Ova, Tnp-Ova and 323-339 Tyr clearly demonstrated that the cytotoxic function of the T hybridoma is strictly correlated with antigen presentation.